Antibody name Company Dilution Anti-p27 Santa Cruz Biotechnology (sc-527) 1:200 Anti- cyclin D1 Sant 1 Supplementary Table 1. List of antibodies used in western blotting Antibody name Company Dilution Anti-p27 Santa Cruz Biotechnology (sc-527) 1:200 Anti- cyclin D1 Santa Cruz Biotechnology (sc-20044) 1:200 Anti-E-cadherin Santa Cruz Biotechnology (sc ...
Antibody Review - Antibody Search Engine polyclonal anti-TGF-beta type I receptor antibody (dilution 1:500; Santa Cruz Biotechnology); rabbit polyclonal anti-Smad7 antibody (dilution 1:500; Santa Cruz Biotechnology; goat polyclonal anti-p-Smad2/3 antibody (dilution 1:500; Santa Cruz ...
Sox-2 antibody detection using Western - SDS-PAGE and Western Blotting I'm using Sox-2 antibody(Y-17): SC-17320, Santa Cruz. Goat polyclonal anti-human. (1:1000 dilution used) Secondary was Rabbit anti-goat IgG HRP, ...
Western (Immuno-) Blotting Santa Cruz Biotechnology, Inc Incubate the blocked membrane in primary antibody diluted in Blotto for 1 hour at room temperature. (For phospho-specific antibodies: Use Blotto B with 0.01% ...
Protocols - Santa Cruz Biotechnology, Inc Santa Cruz Biotechnology, Inc. Antibodies - World Leader in Antibody and siRNA Products. ... Protocol. Copyright ©, 2007-2014, Santa Cruz Biotechnology, Inc.
goat anti-mouse IgG-HRP | Santa Cruz Biotech supplied at 200 µg in 0.5 ml volume; horseradish peroxidase conjugated secondary antibody; to be used in Western blotting at a dilution of 1:500 - 1:5000, use ...
Immunofluorescence Cell Staining Santa Cruz Biotechnology, Inc Santa Cruz Biotechnology, Inc. Antibodies - World Leader in Antibody and siRNA ... following the Formalin-Fixed, Paraffin-Embedded Tissue Sections protocol, ...
goat anti-rabbit IgG-HRP | Santa Cruz Biotech supplied at 200 µg in 0.5 ml volume; horseradish peroxidase conjugated secondary antibody; to be used in Western blotting at a dilution of 1:500 - 1:5000, use ...
Peptide Neutralization Santa Cruz Biotechnology, Inc Determine the highest antibody dilution at which a consistently positive result is achieved for the desired test. For example, H-Ras (259) is recommended for ...
ELISA Assays Santa Cruz Biotechnology, Inc Add test antibody samples and controls at 50 µl/well diluted in blocking buffer. Antibodies may be serially diluted for determining titer or diluted to previously ...
